服務

  • 哪里購買
  • 選擇您的所在地

GPCR單克隆抗體生產

As the largest human membrane protein family responsible for functions such as sensory regulations and cellular responses, GPCRs are considered as one of the most valuable drug targets in pharmaceutical industry. Abnova has developed robust, reliable, and cost-effective high throughput platform of proteoliposome reconstitution system and monoclonal antibody (Mab) production targeting GPCRs to overcome the hindrance of lacking suitable antigen for producing high-quality Mabs.

By optimizing cell-free protein synthesis in the presence of liposomes and purification process via discontinuous sucrose density gradient centrifugation, soluble reconstituted GPCR proteoliposome antigens are synthesized. Abnova’s exclusive techniques in manufacturing proprietary bioreagents allow us to not only produce GPCR proteoliposomes, amenable to functional analysis, but also generate customized Mabs with sensitivity, specificity and wide dynamic range. High successful rate on customized GPCR Mabs has also expanded our field to deliver Mabs targeting various membrane proteins as well.
 
Related FAQ
Technology Comparison for Proteoliposome Expressions

  Conventional Expression System Bilayer / Dialysis Technique Bilyar Automation System
 Stability Low Intermediate High
 Solubility Low Intermediate High
 Yield Low Low High
 Versatility Low Intermediate High
 Throughput Low Low High
 
 
Six Categories and 300+ GPCR Proteoliposomes

  Catalogue Products Made to Order
 Adhesion Class 2 11
 Class A 67 116
 Class B 2 9
 Class C 2 11
 Class Frizzled 2 1
 Others 4 79
 
 
 
 
Demonstrations

Bioactivity Profile on GPCR Protein Synthesized by Bilayer Automation System

Surface Plasmon Resonance (SPR) Analysis of Wildtype DRD1
Ligand-binding activity of DRD1 was determined using SPR in which dopamine and histamine were immobilized onto the measuring cell and reference cell at the same level. Wildtype DRD1 specifically bound to dopamine at KD value of 0.7X10-6 M.

 
 
Characterization of Abnova's Mab Against GPCR Proteins

 

Affinity Analysis of anti-DRD1 Mouse Mabs
Affinity of 36 mouse Mabs were evaluated using Scatchard plot with ELISA. The Kd values of mouse Mab ranged from 10-7 M to 10-10 M, with half of the population showing high sensitivity toward DRD1.

 

Specificity Analysis of GPCR Mouse Mabs
Mouse Mabs against 3 different GPCRs, including GHSR (Class A), PTGER1 (Class A) and T1R1 (Class C), were evaluated using ELISA. All anti-GPCR mAbs showed specific interaction with its intended target antigen.

 
References

Takeda, H., et.al. (2015). Scientific Reports. DOI: 10.1038/srep11333
Goren, M.A. and Fox, B.G. (2008). Protein Expression and Purification. DOI:10.1016/j.pep.2008.08.002
Nozawa, A., et. al. (2016). Plant Cell Physiology. DOI:10.1093/pcp/pcm150
 

如有任何疑問,請聯系 : [email protected]

  • RSS
  • YouTube
  • Linkedin
  • Facebook
江苏十一选五开奖视频 海南琼崖麻将有群进吗 天天捕鱼赢话费鱼百万怎么捕获 手机问道新区怎么赚钱之道 众博彩票安卓 租赁路基铁板赚钱吗 搞果园赚钱不 捕捕鱼来了官网 怎样使用互联网赚钱 卖剪刀赚钱吗 捕鱼达人旧版本下载 天然气改造赚钱吗 国际麻将游戏下载 孕妇在家干什么工作赚钱 买足彩怎么才能赚钱 大通彩票苹果 彩票计划公式能赚钱是真的